The 3′ overhang and no-overhang templates used for in vitro DNA replication reactions were constructed by ligating oligonucleotides to the plasmid vVA25 following its linearization with SapI. To ...

Cells have evolved careful checks to ensure DNA is copied only once, but how they switch on replication at the right moment ...

DNA replication in differentiated cells follows a defined program, but when and how it is established during mammalian development is not known. Here we show using single-cell sequencing, that late ...

DNA, often called the blueprint of life, is built from four chemical building blocks known as deoxy-nucleoside triphosphates (dNTPs)—dATP, dTTP, dGTP, and dCTP. Cells constantly need these molecules ...